Standard Curve

The Standard Curve tab is available for endpoint experiments with standard curve information entered. See how to enter standard curve info here.

Endpoint Graph UI Elements

Number	Element	Description
1	Group Visibility Toggle	Click the checkbox for a group to toggle its visibility in the graph. Wells not assigned to a group can be viewed with the UNGROUPED toggle.
2	Select All	Toggle all groups to be visible on the graph.
3	Select None	Toggle off visibility of all groups.
4	View Select	Toggle between Group Average of the wells assigned to a group or Individual Wells to see each well plotted
5	Export Graph	Export the graph as a .png or .svg file for lab meetings or presentations. .png export size can be selected prior to export. .svg images are vector-based so they scale automatically.
6	Standard Curve Graph	Measured samples are the samples with known concentrations entered in the Endpoint Plate Layout and their measured optical density. Those samples are used to calculate a standard curve using the model selected in the Fit Select. Unknown or blank groups are added to the graph with the concentration calculated from the equation for the standard curve.
7	Fit Select	Choose between Linear Regression and Four-Parameter Logistic Regression (4PL) as the model to fit the measured samples to an equation. This selection should persist the next time the experiment is opened on the same computer.
8	Equation Visibility Toggle	Click to show or hide the equation generated to fit the data. The R2 value denotes how well the curve fits the data with a value close to 1 being a good fit.